anti human tfpi polyclonal ab  (R&D Systems)

Journal: JCI insight

Article Title: Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies.

doi: 10.1172/jci.insight.98459

Figure Lengend Snippet: Figure 2. HETE-PLs enhance thrombin generation in the absence of FIX or FXI, or in the absence of tissue factor pathway inhibitor (TFPI) and FVIII combined. (A and B) Thrombin generation was initiated by addition of liposomes to plasma deficient in FIX, as described in Methods. Liposomes were generated as described in Methods, and 10% 1-stearoyl-2-arachidonyl-PE (SAPE) was replaced with 10% 15-HETE-PE where indicated. (C and D) Thrombin generation was initiated by addition of liposomes to plasma deficient in FXI, as described in Methods. Liposomes were generated as described in Methods, and 10% SAPE was replaced with 10% 15-HETE-PE where indicated. (A–D) n = 3, mean ± SEM, *P < 0.05. A representative trace is shown for each. (E–H) Thrombin generation was initiated by direct addition of liposomes to plasma as described in Methods, at 10% HETE-PE, and measured in the absence (E and G) or presence (F and H) of 100 nmol/l anti-TFPI antibody preincubated for 15 minutes, in either normal (E and F) of FVIII-deficient plasma (G and H). (I–L) Thrombin generation was initiated by direct addition of liposomes to plasma as described in Methods, at 10% HETE-PC, and measured in the absence (I and K) or presence (J and L) of 80 nmol/l anti-TFPI antibody preincubated for 15 minutes, in either normal (I and J) of FVIII-deficient plasma (K and L). Data is shown as Tukey box plots, with 1-way ANOVA with Tukey post hoc multicomparison test. *P < 0.05.

Article Snippet: Anti–human-TFPI goat polyclonal antibody (catalog AF2974) was from R&D Systems.

Techniques: Liposomes, Clinical Proteomics, Generated

Journal: JCI insight

Article Title: Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies.

doi: 10.1172/jci.insight.98459

Figure Lengend Snippet: Figure 3. HETE-PL restoration of hemostasis in mice lacking FVIII and enhancement of thrombin generation in FVIII deficiency, with/without tissue factor pathway inhibitor (TFPI) inhibition. (A and B) Summary data for thrombin generation in plasma as shown in Figure 3, E–L (n = 3, mean ± SEM). **P < 0.01, compared with control, as determined by 1-way ANOVA and post hoc Tukey tests. (C and D) 12-HETE-PE restores hemostasis in mice lacking FVIII. Male mice (11 weeks) were administered 78 ng HETE-PE in liposomes as described in Methods before a 3-mm tail cut. Bleeding time and Hb loss were determined as in Methods (n = 4–9 mice per group). Data is shown as Tukey box plots, with 1-way ANOVA with Tukey post hoc multicomparison test. *P < 0.05, ***P < 0.005, data shown as squares in box plot are outliers (values 1.5–3 times lower or higher than the interquartile range).

Article Snippet: Anti–human-TFPI goat polyclonal antibody (catalog AF2974) was from R&D Systems.

Techniques: Inhibition, Clinical Proteomics, Control, Liposomes